Friday, April 19, 2019

Happy Easter - Lupine publishers

May this Easter bring lots of happiness to your life and make all your wishes come true! Wishing you health, prosperity, longevity and peace this Easter and always. And wish you a very Happy Easter.

Monday, April 15, 2019

Journal of Clinical and Medical Case Studies - Lupine publishers

There is currently no validated micro(mi)RNA diagnostic stool test to screen for colon cancer (CC) on the market because of the complexity of fecal density, vulnerability of stool to daily changes, and the presence of three sources of miRNAs in stool (cell-free from fecal homogenates, exsosomal miRNAs from fecal exosomes, and fecal colonocytes). By employing earlier on a microarray miRNA experiment, using Affymetrix GeneChip miRNA 2.0 Arrays, on immunocaptured and enriched stool colonocytes of 15 subjects [three healthy controls and twelve colon cancer patients [three TNM stage 0-1 (e.g., polyps  1 cm, villous or tubvillous, or with high grade dysplasia), three stage 2, three stage 3, and three stage 4] in triplicates, this allowed for selection of a smaller panel of 14 preferentially expressed mature miRNAs associated with colon cancer (12 Up-Regulated, miR-19a, miR-20a, miR-21, miR-31, miR-34a, miR-96, miR-106a, miR-133a, miR-135b, miR-206, miR-224 and miR-302; and 2 Down-Regulated, miR-143 and miR-145). Then carrying out an absolute quantitative digital PCR on these 15 stool samples from TNM stages 0-4 on total small RNA extracted by immunocapture, followed by RT that employed a Custom TaqMan® miRNA Reverse Transcription (RT) Kit and TaqMan RT Primer Pool, and absolute quantification of miRNAs, in copies/μl, measured using a chip-based Absolute QuantStudio 3D Digital PCR analysis, allowed for validating the microarray results. To ensure that human and not bacterial small total RNA was chosen, coextraction protocols with E. coli K1 strain RS18 was carried out, followed by comparing Agilent electrophoretic patterns with human and bacterial electrophoretic patterns, and also random samples were sequenced using mRNA/miRNA sequencing, to ensure that human and not bacterial mRNA was chosen.

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Monday, April 8, 2019

Medical Case study Journal- lupine publishers

A 11-year-old girl was referred by the Orthopaedic team with swelling of her right knee which started five months back. She had no systemic symptoms or preceding trauma or infection. She was seen by the Orthopaedic team, who aspirated the joint and injected it with steroids, once the cultures came back as negative. She had a good response, although the swelling returned after 8-10 weeks, associated with some stiffness. She was previously fit and well except for visual impairment in her left eye due to Persistent Hyperplastic Primary Vitreous (PHPV). Her examination revealed moderate effusion in her right knee. Her blood tests including inflammatory markers were normal. With a diagnosis of oligoarticular JIA, she had a further intra-articular steroid injection with good response lasting 4 months. Subsequently she required two more steroid injections in the same joint within six months, at which stage she was commenced on weekly Methotrexate injections at 15mg/m2. Despite that, the swelling recurred, which did not respond to intra-articular steroid injection, following which MRI scan was requested. This showed diffuse synovial proliferation with relatively low signal components in some areas, suggestive of diffuse PVNS (Figure 1). Her immunosuppression was stopped, and she was referred for synovectomy.

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Monday, April 1, 2019

Journal of Clinical and Medical Case Studies - lupine publishers

Quality of health service is measured based on structure, process and outcome elements and has multidimensional concept that has been defined in various ways. It is an important aspect of health service delivery system and must be given priority in most countries.A facility based cross sectional quantitative and qualitative study design was employed from January- May 2016 on 396 guardians of under five children. The data were collected using structured exit interviews, facility assessment checklists and record reviews. Data was cleaned and entered Microsoft office Access, EPI info and analyzed by Statistical Package for the Social Science. Binary and multivariate logistic regression was used to identify factors of guardians’ satisfaction and odds ratios, 95% CI and p-value was computed to measure the presence and strength of associations.The study showed that health centers and health posts on average fulfilled 32.4% of the health human power need and 44.1% of major equipment requirement against the national standards. The average adherence of health professional with guidelines of pneumonia and diarrhea managements was 76% and 65.6% respectively. Malnutrition was inappropriately managed and alarming in all components which needs program interventions. The composite average satisfaction level of guardians was 54.3%. Adequacy of information given about child health service (OR=0.49, 95% CI, =0.27, 0.91), expectation about the health services (OR=0.46, 95% CI, = 0.23, 0.91), privacy given during treatments (OR=0.21, 95% CI, = 0.08, 0.54) and preference of health institutions were the predictor variables (OR=0.18, 95% CI, = 0.09, 0.35) for under five child health service satisfactions of guardians.The overall quality of health service was low compared to studies done at different areas on quality of health care. The health institutions were facing shortages of human power, material resources and low adherence with standard treatment guidelines especially on diarrhea and malnutrition managements. The responsible bodies of health sectors at different levels should take actions to improve institutional capacity and performance of care providers in order to improve quality of health services. There should also be continuous monitoring systems to health facilities focused on health care services to strengthen institutional capacity and performance of care providers.

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Monday, March 25, 2019

Journal of Surgery - lupine publishers

A 9-year-old girl presented with 4-month history of swelling and discoloration on the right upper eyelid and inability to open the eyes completely (Figure 1). She was previously fit and well with no past medical history of note. She was initially diagnosed with periorbital cellulitis at the local hospital and was treated with antibiotics and antihistamines for four weeks with no improvement. Initial investigations showed elevated platelet count of 508, and raised inflammatory markers - ESR of 67 mm/hr and CRP of 40 mg/L. The CT scan was suggestive of a mass lesion on the upper eyelid (Figure 2). Biopsy done by the ophthalmology team showed soft tissue fibro-inflammatory mass with reactive lymphoid aggregates and dense histiocytic and eosinophilic inflammation centred around variable calibre vessels. No atypical leukemic/ lymphoid infiltrates were seen. Histiocytic cells were positive for CD160 and negative for Langerin. Hence a diagnosis of small vessel vasculitis was suggested. The child was then referred to our tertiary pediatric rheumatology service.

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Happy Easter - Lupine publishers

May this Easter bring lots of happiness to your life and make all your wishes come true! Wishing you health, prosperity, longevity ...