Abstract
Studies were conducted on valves from a valve bank, frozen for up to 19 years. Obtained results were compared to studies of fresh, non-frozen valves. Stereomicroscopy, polarized light microscopy, scanning microscopy and X-ray diffractometry were used in the studies. It was determined that the structures of valve leaflets after prolonged freezing time (4-5 years) do not return to their initial, pre-frozen state. Changes in the structures after freezing time of over 5 years may, when the valves are implemented, favor faster damage processes, including calcification. Hence it appears beneficial to only implant valves that have been frozen for less than 5 years.
Keywords:Heart Valves; Freezing
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